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KMID : 0545120140240050675
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Journal of Microbiology and Biotechnology
2014 Volume.24 No. 5 p.675 ~ p.682
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Production of α- and β-Galactosidases from Bifidobacterium longum subsp. longum RD47
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Han Yoo-Ri
Youn So-Youn
Ji Geun-Eog
Park Myeong-Soo
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Abstract
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Approximately 50% of people in the world experience abdominal flatulence after the intake of foods containing galactosides such as lactose or soybean oligosaccharides. The galactoside hydrolyzing enzymes of α- and β-galactosidases have been shown to reduce the levels of galactosides in both the food matrix and the human gastrointestinal tract. This study aimed to optimize the production of α- and β-galactosidases of Bifidobacterium longum subsp. longum RD47 with a basal medium containing whey and corn steep liquor. The activities of both enzymes were determined after culturing at 37oC at pH 6.0 for 30 h. The optimal production of α- and β-galactosidases was obtained with soybean oligosaccharides as a carbon source and proteose peptone no. 3 as a nitrogen source. The optimum pH for both α- and β-galactosidases was 6.0. The optimum temperatures were 35oC for α-galactosidase and 37oC for β- galactosidase. They showed temperature stability up to 37oC. At a 1 mM concentration of metal ions, CuSO4 inhibited the activities of α- and β-galactosidases by 35% and 50%, respectively. On the basis of the results obtained in this study, B. longum RD47 may be used for the production of α- and β-galactosidases, which may reduce the levels of flatulence factors.
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KEYWORD
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Bifidobacteria, α- and β- galactosidases, Soybean oligosaccharides, Proteose peptone no. 3, Whey and corn steep liquor
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